Archives on the previous training courses

<<FY2016>>

Training for the cultivation and preservation of anaerobes

Overview   :
  The course is to learn and practice expertise in cultivation and preservation of anaerobes.
Host Division   :    Microbe Division: JCM
Dates   :    November 14 to 15, 2016     2 consecutive days Microbe Division
Number of trainees   :    4 people


Training course for cryopreservation of mouse sperm and embryos
- BRC-new-ovulation method and rapid production of next generation mice -

Overview   :
  We will provide hands-on training of basic laboratory techniques to produce and maintain specific mouse strains, including highly efficient superovulation, in vitro fertilization, cryopreservation of mouse sperm and embryos. We will also demonstrate micro-insemination operation and nuclear-transplantation cloning.
Host Division   :    Bioresource Engineering Division
Dates   :    From October 25 to 28, 2016     4 consecutive days
Number of trainees   : 4 people


Training course for basic technologies required for Arabidopsis research
Optional course: Training course for cultivation of Brachypodium distachyon Bd21

Overview   :
  This is training course for the researcher who is going to study the plant science using Arabidopsis.
  <Optional course (at your request)>
  Trainees will learn the cultivation procedure for Brachypodium distachyon, a model of monocot plants.
Host Division   :    Experimental Plant Division
Dates   :    From August 25 to 26 AM, 2016   <Optional course> August 26 PM, 2016
Number of trainees   : 4 people (Option 3 people)


Technical training course for maintenance and transformation of Arabidopsis T87 cells
Optional course: Technical training course for the cryopreservation of tobacco BY-2 cells

Overview   :
  This course presents basic methods to maintain Arabidopsis T87 suspension cultured cells (day 1)and applied methods for protoplast transient assay systems (day 2).
  <Optional course (at your request)>
  Trainees will learn the basis and procedure for cryopreservation of plant cultured cells in liquid nitrogen.
Host Division   :    Experimental Plant Division
Dates   :    From August 22 to 23 AM, 2016   <Optional course> From August 23 PM to 24 AM, 2016
Number of trainees   : 5 people (Option 3 people)


The Modified SHIRPA Technical Training Course

Overview   :
  The Modified SHIRPA is one of the simple and quick methods for evaluating comprehensive mouse phenotypic traits on mouse morphology, behavior, sensory responses, etc.
  It includes approximately 60 test items for visual inspection by manual handling using simple equipment (refer to the RIKEN SHIRPA homepage http://shirpa.brc.riken.jp/). It is a globally standardized set of procedures specified by the International Mouse Phenotyping Consortium (IMPC http://www.mousephenotype.org/).
  The Technical Training Course navigates this method through lecture, demonstration, and laboratory practices so that trainees can master it in 5 days.
Host Division   :    Technology and Development Team for Mouse Phenotype Analysis: Japan Mouse Clinic
Dates   :    May 23 to 27, 2016, 2016     5 consecutive days
Number of trainees   : 1 people


<<FY2015>>

The Modified SHIRPA Technical Training Course

Overview   :
The Modified SHIRPA is one of the simple and quick methods for evaluating comprehensive mouse phenotypic traits on mouse morphology, behavior, sensory responses, etc.
  It includes approximately 60 test items for visual inspection by manual handling using simple equipment (refer to the RIKEN SHIRPA homepage http://shirpa.brc.riken.jp/). It is a globally standardized set of procedures specified by the International Mouse Phenotyping Consortium (IMPC http://www.mousephenotype.org/).
  The Technical Training Course navigates this method through lecture, demonstration, and laboratory practices so that trainees can master it in 5 days.
Host Division   :    Technology and Development Team for Mouse Phenotype Analysis: Japan Mouse Clinic
Dates   :    February 15 to 19, 2016     5 consecutive days
Number of trainees   : 1 people


Lecture and demonstration for handling recombinant adenovirus

Overview   :
Lecture and practical demonstration about handling recombinant adenovirus, including the construction of adenoviral shuttle vector, the production of viral particle and the methods for purification of recombinant virus.
Host Division   :    Gene Engineering Division
Dates   :    December 21 to 22, 2015     2 consecutive days
Number of trainees   : 4 people


Technical training on basic handling of aerobic bacteria and 16S rRNA gene sequence analysis

Overview   :
This course presents basic experimental methods to revive aerobic bacteria from freeze-dried ampoule and to determine a 16S rRNA gene sequence.
Host Division   :    Microbe Division, JCM
Dates   :    December 10 to 11, 2015     2 consecutive days
Number of trainees   : 3 people


Technical training course for ICSI (intracytoplasmic sperm injection) of mice

Overview   :
Technical training course for mouse ICSI (intracytoplasmic sperm injection) by Piezo-assisted micromanipulation.
Host Division   :    Bioresource Engineering Division
Dates   :    November 16 to 18, 2015     3 consecutive days
Number of trainees   : 3 people


Training course for cultivation of Brachypodium distachyon Bd21

Overview   :
Trainees will learn the cultivation and transformation procedure for Brachypodium distachyon, a model of monocot plants.
Host Division   :    Experimental Plant Division
Dates   :    November 10 to 11, 2015     2 consecutive days
Number of trainees   : 4 people


"Lecture and practice on cryopreservation of human iPS cells (vitrification method)" FY2015

Overview   :
   All human iPS cells (including iPS cells derived from patients) are preserved with a vitrification method in our repository. The procedures of the vitrification method are specific and quite different from the procedures of typical freezing method for ordinary cell lines. Consequently, the thawing procedures for the cells preserved with the vitrification method are also quite different from the procedures of typical thawing method for ordinary cell lines. In this course, we will offer a lecture on how to preserve human iPS cells with a vitrification method and how to thaw them. We do not deny that human iPS cells can be preserved with ordinary freezing methods. However, the efficiencies of preservation differ very much among cell lines with ordinary freezing methods. By contrast, the efficiencies do not differ so much with the vitrification method we are using.
   We do not deny that human iPS cells can be preserved with ordinary freezing methods. However, the efficiencies of preservation differ very much among cell lines with ordinary freezing methods. By contrast, the efficiencies do not differ so much with the vitrification method we are using.
Host Division   :    Cell Engineering Division
Dates   :    September 4, 2015     One day
Number of trainees   : 2 people


Course A : This is training course for the researcher who is going to study the plant science using Arabidopsis
Course B : Trainees will learn the basis and procedure for cryopreservation of plant cultured cells in liquid nitrogen

Overview   :
<Course A> Trainees will learn the cultivation and transformation procedure for Brachypodium distachyon, a model of monocot plants.
<Course B> Trainees will learn the basis and procedure for cryopreservation of plant cultured cells in liquid nitrogen.
Host Division   :    Experimental Plant Division
Dates   :    August 26 to 27, 2015     2 consecutive days
Number of trainees   : 2 people


Technical training course for maintenance and transformation of Arabidopsis T87 cells

Overview   :
This course presents basic methods to maintain Arabidopsis T87 suspension cultured cells (day 1)
and applied methods for protoplast transient assay systems (day 2).
Host Division   :    Experimental Plant Division
Dates   :    August 24 to 25, 2015     2 consecutive days
Number of trainees   : 4 people


<<FY2014>>

The couse of basic technologies for cell culture; Couse II

Overview   :    Only in Japanese
Host Division   :    Cell Engineering Division Co-host The Japanese Tissue Culture Association (Japanese text only)
Dates   :    January 31 to February 1, 2015   2 consecutive days
Number of trainees   :    8 people


Training course for basic techniques for handling yeasts

Overview   :    Only in Japanese
Host Division   :    Microbe Division, JCM
Dates   :    January 29 to 30, 2015   2 consecutive days Microbe Division
Number of trainees   :    1 person


Training course for cryopreservation of mouse sperm and embryos

Overview   :    We will provide hands-on training of basic laboratory techniques to produce and maintain specific mouse strains, including in vitro fertilization as well as cryopreservation of mouse sperm and embryos. We will also demonstrate micro-insemination operation and nuclear-transplantation cloning.
Host Division   :    Bioresource Engineering Division
Dates   :    October 6 to 9, 2014   4 consecutive days
Number of trainees   :    3 people


"Lecture and practice on cryopreservation of human iPS cells (vitrification method)" FY2014

Overview   :
   All human iPS cells (including iPS cells derived from patients) are preserved with a vitrification method in our repository. The procedures of the vitrification method are specific and quite different from the procedures of typical freezing method for ordinary cell lines. Consequently, the thawing procedures for the cells preserved with the vitrification method are also quite different from the procedures of typical thawing method for ordinary cell lines. In this course, we will offer a lecture on how to preserve human iPS cells with a vitrification method and how to thaw them.
   We do not deny that human iPS cells can be preserved with ordinary freezing methods. However, the efficiencies of preservation differ very much among cell lines with ordinary freezing methods. By contrast, the efficiencies do not differ so much with the vitrification method we are using.
Host Division   :    Cell Engineering Division
Dates and Number of trainees   :
   2 people, May 16, 2014 (One day)
   2 people, July 4, 2014 (One day)
   2 people, September 5, 2014 (One day)


Course A : Training course for cultivation and transformation of Brachypodium distachyon Bd21
Course B : Technical training course for the cryopreservation of tobacco BY-2 cells

Overview   :
<Course A> Trainees will learn the cultivation and transformation procedure for Brachypodium distachyon, a model of monocot plants.
<Course B> Trainees will learn the basis and procedure for cryopreservation of plant cultured cells in liquid nitrogen.
Host Division   :    Experimental Plant Division
Dates   :    September 1 to 2, 2014     2 consecutive days
Number of trainees   :
Course A : 3 people
Course B : 1 person


Course A: Technical training course for maintenance and transformation of Arabidopsis T87 cells
Course B: Training course for basic technologies required for Arabidopsis research

Overview   :    Only in Japanese
Host Division   :    Experimental Plant Division
Dates   :    August 25 to 26, 2014     2 consecutive days
Number of trainees   :
nbsp; Course A : 2 people
nbsp; Course B : 1 person


<<FY2013>>

Training course for expression and functional analysis of recombinant proteins
~ Gene expression of biomass-degrading enzymes in E. coli ~

Overview   :    Only in Japanese
Host Division   :    Gene Engineering Division
Dates   :    February 18 to 19, 2014   2 consecutive days Gene Engineering Division
Number of trainees   :    3 people


Announcement of technical training on how to handle human embryonic stem cells

Overview   :    Only in Japanese
Host Division   :    Cell Engineering Division
Dates :
   June 7, 2013 (One day)
   October 4, 2013 (One day)
   February 7, 2014 (One day)
Number of trainees   :    2 people


Training course on DNA-DNA hybridization for microbial classification and identification

Overview   :    Only in Japanese
Host Division   :    Microbe Division, JCM
Dates   :    February 6 to 7, 2014   2 consecutive days Microbe Division
Number of trainees   :    6 people


Lecture and demonstration for handling recombinant adenovirus

Overview   :    Only in Japanese
Host Division   :    Gene Engineering Division
Dates   :    December 16 to 17, 2013   2 consecutive days Gene Engineering Division
Number of trainees   :    9 people


Technical training course for ICSI (intracytoplasmic sperm injection) of mice

Overview   :    Only in Japanese
Host Division   :    Bioresource Engineering Division
Dates   :    November 25 to 27, 2013    3 consecutive days
Number of trainees   :    3 people Bioresource Engineering Division


"Lecture and practice on cryopreservation of human iPS cells (vitrification method)" FY2013

Overview   :
   All human iPS cells (including iPS cells derived from patients) are preserved with a vitrification method in our repository. The procedures of the vitrification method are specific and quite different from the procedures of typical freezing method for ordinary cell lines. Consequently, the thawing procedures for the cells preserved with the vitrification method are also quite different from the procedures of typical thawing method for ordinary cell lines. In this course, we will offer a lecture on how to preserve human iPS cells with a vitrification method and how to thaw them.
   We do not deny that human iPS cells can be preserved with ordinary freezing methods. However, the efficiencies of preservation differ very much among cell lines with ordinary freezing methods. By contrast, the efficiencies do not differ so much with the vitrification method we are using.
Host Division   :    Cell Engineering Division
Dates and Number of trainees   :
   2 people, May 10, 2013 (One day)
   3 people, July 5, 2013 (One day)
   1 people, November 1, 2013 (One day)


Technical training course for the cryopreservation of tobacco BY-2 cells

Overview   :    Trainees will learn the basis and procedure for cryopreservation of plant cultured cells in liquid nitrogen.
Host Division   :    Experimental Plant Division
Dates   :    August 26 to 27, 2013     2 consecutive days Experimental Plant Division
Number of trainees   :    2 people


Training course for cultivation of Brachypodium distachyon Bd21

Overview   :    Trainees will learn the cultivation procedure for Brachypodium distachyon, a model of monocot plants.
Host Division   :    Experimental Plant Division
Dates   :    August 6, 2013     1 day Experimental Plant Division
Number of trainees   :    1 person


<<FY2012>>

The Modified SHIRPA technical training course

Overview   :
   The Modified SHIRPA is one of the simple and quick methods for evaluating comprehensive mouse phenotypic traits on mouse morphology, behavior, sensory responses, etc.
It includes approximately 60 test items for visual inspection by manual handling using simple equipment (refer to the RIKEN SHIRPA homepage http://shirpa.brc.riken.jp/). It is a globally standardized set of procedures specified by the International Mouse Phenotyping Consortium (IMPC http://www.mousephenotype.org/).
The Technical Training Course navigates this method through lecture, demonstration, and laboratory practices so that trainees can master it in 5 days.

Host Division   :    Technology and Development Team for Mouse Phenotype Analysis: Japan Mouse Clinic
Dates   :    March 25 to 29, 2013     5 consecutive days
Number of trainees   :    2 people


Lecture and practice on cryo-preservation of human iPS cells (vitrification method)

Overview   :    Only in Japanese
Host Division   :    Cell Engineering Division
Dates and Number of trainees   :
   2 people, September 7, 2012 (One day)
   1 people, November 2, 2012 (One day)
   4 people, January 11, 2013 (One day) Cell Engineering Division
   2 people, March 1, 2013 (One day)


Training for the cultivation and preservation of anaerobes

Overview   :    Only in Japanese
Host Division   :    Microbe Division: JCM
Dates   :    February 25 to 26, 2013     2 consecutive days Microbe Division
Number of trainees   :    4 people


Technical training course for Western blot analysis

Overview   :    Only in Japanese
Host Division   :    Gene Engineering Division
Dates   :    November 15 to 16, 2012     2 consecutive days Gene Engineering Division
Number of trainees   :    7 people


Training course for cryopreservation of mouse sperm and embryos

Overview   :
   We will provide hands-on training of basic laboratory techniques to produce and maintain specific mouse strains, including in vitro fertilization as well as cryopreservation of mouse sperm and embryos. We will also demonstrate micro-insemination operation and nuclear-transplantation cloning.

Host Division   :    Bioresource Engineering Division
Dates   :    October 9 to 12, 2012     4 consecutive days Bioresource Engineering Division
Number of trainees   :    3 people


Technical training course for the cryopreservation of tobacco BY-2 cells

Overview   :
   Trainees will learn the basis and procedure for cryopreservation of plant cultured cells in liquid nitrogen.
Host Division   :    Experimental Plant Division
Dates   :    September 10 to 11, 2012     2 consecutive daysExperimental Plant Division
Number of trainees   :    3 people


Course A: Technical training course for maintenance and transformation of Arabidopsis T87 cells
Course B: Training course for basic technologies required for Arabidopsis research

Overview   :    Only in Japanese
Host Division   :    Experimental Plant Division
Dates   :    September 3 to 4, 2012     2 consecutive days
Number of trainees   :
   Course A : 3 people
   Course B : 6 people


The Modified SHIRPA Technical Training Course

Overview   :
   The Modified SHIRPA is one of the simple and quick methods for evaluating comprehensive mouse phenotypic traits on mouse morphology, behavior, sensory responses, etc. It includes approximately 60 test items for visual inspection by manual handling using simple equipment (refer to the RIKEN SHIRPA homepage http://shirpa.brc.riken.jp/). It is a globally standardized set of procedures specified by the International Mouse Phenotyping Consortium (IMPC http://www.mousephenotype.org/). The Technical Training Course navigates this method through lecture, demonstration, and laboratory practices so that trainees can master it in 5 days.

Host Division   :    Technology and Development Team for Mouse Phenotype Analysis: Japan Mouse Clinic
Dates   :    July 30 to August 3, 2012     5 consecutive days
Number of trainees   :    4 people