Human iPS cells cannot be cultured with ordinary culture method and it requires special skills. This course provides a lecture and practical training for the method of passaging human iPS cells with feeder cells.
All human iPS cells (including iPS cells derived from patients) are preserved with a vitrification method in our repository. The procedures of the vitrification method are specific and quite different from the procedures of typical freezing method for ordinary cell lines. Consequently, the thawing procedures for the cells preserved with the vitrification method are also quite different from the procedures of typical thawing method for ordinary cell lines. In this course, we will offer a lecture on how to preserve human iPS cells with a vitrification method and how to thaw them in 1-day course.
We don't deny that human iPS cells can be preserved with ordinary freezing methods. However, the efficiencies of preservation differ very much among cell lines with ordinary freezing methods. By contrast, the efficiencies don't differ so much with the vitrification method we are using.
Lecture and practice on cryopreservation of human iPS cells (vitrification method)
Lecture and practice on Subculture of human iPS cells
1) May 19, 2017 (1 day)
2) July 7, 2017 (1 day)
3) September 8, 2017 (1 day)
4) November 10, 2017 (1 day)
5) January 19, 2018 (1 day)
6) March 2, 2018 (1 day)
The same content will be presented on each day.
BioResource Building for Cell Research, RIKEN BioResource Center (Tsukuba)
Researchers and technicians of relevant backgrounds from academia, governments, and industries.
6 people in total
Please download, fill out, and submit the application form with an appendix including required information to the secretariat by post (see "application procedures").
* Please ask the person holding an administrative position to sign in the "Responsible person" column in the application form.
1) April 21, 2017 Application closed.
2) Jun 9, 2017 Application closed.
3) August 4, 2017 Application closed.
4) October 13, 2017
5) December 15, 2017
6) February 2, 2018
The application must reach us no later than above each deadline.
We may stop accepting applications earlier when the number of applicants reaches our maximum capacity.
Training course for human iPS cells
Secretariat of the technical courses at RIKEN Tsukuba Institute
3-1-1 Koyadai,Tsukuba-shi, Ibaraki 305-0074 Japan