All human iPS cells (including iPS cells derived from patients) are preserved with a vitrification method in our repository. The procedures of the vitrification method are specific and quite different from the procedures of typical freezing method for ordinary cell lines. Consequently, the thawing procedures for the cells preserved with the vitrification method are also quite different from the procedures of typical thawing method for ordinary cell lines. In this course, we will offer a lecture on how to preserve human iPS cells with a vitrification method and how to thaw them.
We do not deny that human iPS cells can be preserved with ordinary freezing methods. However, the efficiencies of preservation differ very much among cell lines with ordinary freezing methods. By contrast, the efficiencies do not differ so much with the vitrification method we are using.
1) May 15, 2015 (1 day)
2) July 3, 2015 (1 day)
3) September 4, 2015 (1 day)
4) November 6, 2015 (1 day)
5) January 15, 2016 (1 day)
6) March 4, 2016 (1 day)
BioResource Building for Cell Research, RIKEN BioResource Center (Tsukuba)
Researchers and technicians of relevant backgrounds from academia, governments, and industries.
Please download, fill out, and submit the application form with an appendix including required information to the secretariat by post (see "application procedures").
* Please ask the person holding an administrative position to sign in the "Responsible person" column in the application form.
Lecture and practice on cryopreservation of human iPS cells (vitrification method)
Secretariat of the technical courses at RIKEN Tsukuba Institute
3-1-1 Koyadai,Tsukuba-shi, Ibaraki 305-0074 Japan